He moderately stained neurons from the medial and NS-018 lateral habenular nuclei(Fig 1J, MHb, LHb) within the epithalamus. Far more strongly stained neurons were located in the mediodorsal, lateral dorsal, and ventral lateral thalamic nuclei (Fig 1J, MD, LD, VL) as well as the reuniens thalamic nucleus(Fig 1J, Re). Scattered lightly to moderately stained neurons were identified inside the location in the globus pallidus(Fig 1J, GP). The cells with the lateral hypothalamic nucleus(Fig 1J, LH; Fig 2K) exhibited moderate to robust staining and were more densely arrayed. 3.three Prosencephalon Beginning in the forebrain level the distribution of TCF7L2-labeled cells integrated the robustly stained neurons from the subfornical organ(Fig 1K, SFO; Fig 2L), these of the lateral preoptic region(Fig 1K, LPO; Fig 3A), the medial preoptic nucleus(Fig 1K, MPO; Fig 3B) and smaller sized nuclei including the nucleus of horizontal limb of diagonal band(Fig 1K, DBh),J Chem Neuroanat. Author manuscript; accessible in PMC 2013 October 01.NIH-PA Author Manuscript NIH-PA Author Manuscript NIH-PA Author ManuscriptWeaver et al.Pageaccumbens nucleus(Fig 1K, Acb) and magnocellular preoptic nucleus(Fig 1K, MCPO). At the remaining levels, intensely labeled TCF7L2 cells composed quite a few layers lining the ventricular and subventricular zones from the lateral ganglionic eminence(Fig 1L, LG) which type the septal(Fig 1L, Sn, Fig PubMed ID:http://www.ncbi.nlm.nih.gov/pubmed/21237502 3C) and striatal neuroepithelium. Despite the fact that present in the exact same zones on the lateral ganglionic eminence forming cortical neuroepithelium(Fig 1L, Cn) and medial ganglionic eminence forming the striatal neuroepithelium(Fig 1L, Mge), the cells of this layer exhibited considerably much less intense labeling for TCF7L2. The strongest expression of TCF7L2 in the neuroepithelium was identified involving E14 and E18.5. A handful of moderately stained and scattered cells were found within the medial septal nucleus(Fig 1L, MS). 3.4 Parasagittal Planes Parasagittal sections provided further insight towards the distribution and expression of TCF7L2. The robust staining in the dense collection of neurons shown in Fig 3D-E which compose the parafascicular(PF), mediodorsal(MD), subparafascicular(SPF), anteriomedial(AM), ventral medial(VM), ventral posterior medial(VPM), and reticular(Ret) thalamic nuclei too because the unstained fibers from the fasciculus retroflexus(fr) above as well as the cells in the zona incerta(ZI) beneath contributed to the well-defined demarcation of thalamic boundaries in the pretectum above as well as the hypothalamus beneath. This sagittal section also illustrates labeled TCF7L2 cells from the tectum such as moderately labeled cells with the pretectum(Fig 3D-E, Ptec), periaqueductal gray(Fig 3D, PAG), dorsomedial periaqueductal gray(Fig 3D, DMPAG) and superior colliculus(Fig 3D, SC) at the same time as cells on the epithalamus including posterior commissural(pc), precommissural(PrC) and the medial and lateral habenular nuclei(Fig 3E, MHb, LHb) and the ventrolateral periaqueductal gray area(Fig 3D, VLPAG). In Fig 3F, moving subthalamically a clear profile of robust TCF7L2 labeled cells is usually seen composing the ventromedial hypothalamic nucleus(VMH) near the pituitary(P) within this parasagittal section near the midline. In the brain stem adjacent towards the thalamus the reticular cells of the pons were found to exhibit a robust immunoreactive label for TCF7L2(Fig 3F, RFp). This was found to become characteristic on the reticular cells throughout the brain stem including those reticular cells from the medulla(Fig 3F, RFm) and the gigantocellular r.
