To decide whether or not peptides from HCV core protein made up of amino acids 166 are able of binding to DDX3X in cells, HuH-seven cells have been transiently transfected with vectors that categorical GFP or GFP fusions to HCV main peptides from amino acids 166
In DDX3X rescue experiments, 3 mg of plasmid encoding mCherry or DDX3X-mCherry fusions (pmCherry-N1...